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  • "Connecting the dots: linking PAMP perception to receptor kinase-mediated immune outputs"
    relies on the recognition of microbes via the perception of pathogen associated molecular patterns PAMPs by surface localized receptors called pattern recognition receptors PRRs The Arabidopsis leucine rich repeat receptor kinases LRR RKs FLS2 and EFR are the PRRs for bacterial flagellin or flg22 and elongation factor Tu or elf18 respectively Within seconds of PAMP binding FLS2 and EFR form a ligand induced complex with the regulatory LRR RK SERK3 BAK1 leading to phosphorylation of both proteins FLS2 and potentially EFR also forms a constitutive complex with the membrane associated cytoplasmic kinase BIK1 that get phosphorylated in a BAK1 dependent manner upon PAMP binding BIK1 is a positive regulator of several FLS2 and EFR mediated responses FLS2 and EFR activation leads to several immune responses including bursts of Ca2 and reactive oxygen species ROS activation of mitogen activated protein kinases MAPKs and calcium dependent protein kinases CDPKs and transcriptional reprogramming ultimately leading to PAMP triggered immunity The mechanisms controlling PRR activation at the plasma membrane and regulating intracellular signaling remain however largely unknown Here I will present recent work illustrating how activated PRR complexes at the plasma membrane directly engage with downstream signaling and how these events are tightly regulated

    Original URL path: http://www.psb.vib-ugent.be/seminars/icalrepeat.detail/2015/04/10/149/-/connecting-the-dots-linking-pamp-perception-to-receptor-kinase-mediated-immune-outputs (2016-04-26)
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  • "BGLU6, a single trait locus affecting flavonol accumulation in Arabidopsis thaliana natural variations"
    Save ical Send to Google Calendar Send to Yahoo Calendar Save ical Preserve formatting in description only supported in some calendar applications Close Monday 20 April 2015 02 00pm 03 30pm ABSTRACT Flavonols are colourless secondary metabolites primarily regarded as UV protection pigments which are deposited in plants in their glycosylated forms The glycosylation of flavonols is catalysed by UDP sugar dependent glycosyltransferases UGTs Although the structures of flavonol glycosides accumulating in A thaliana is known many genes involved in the avonol glycosylation pathway are yet to be discovered The flavonol glycoside profiles of seedlings from 79 naturally occuring A thaliana accessions were screened using high performance thin layer chromatography A qualitative variation in flavonol 3 O gentiobioside 7 O rhamnoside F3GG7R content was identified Using recombinant inbred lines the F3GG7R trait was mapped and identified as glycoside hydrolase family 1 type gene BGLU6 that encodes a homolog of acyl glucose dependent glycosyl transferases and being co expressed with genes linked to phenylpropanoid biosynthesis Location Jozef Schell seminar room Contact Dr Ralf Stracke Bielefeld University nDept of Biology nBielefeld nGERMANY Back PSB Missions The Department Infrastructure Core Facilities International Projects Publications News Seminars About PSB PSB Missions The Department Infrastructure

    Original URL path: http://www.psb.vib-ugent.be/seminars/icalrepeat.detail/2015/04/20/150/-/bglu6-a-single-trait-locus-affecting-flavonol-accumulation-in-arabidopsis-thaliana-natural-variations (2016-04-26)
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  • "Genetic and epigenetic components in plant growth and crop yield"
    extent in specific environmental conditions we use mutational analysis and epiline selection to identify novel genes or networks that function in growth and yield A forward genetics program in Arabidopsis used leaf growth mutants to identify novel genes in plant growth The chromatin state at RNA PII transcript elongation is an important regulatory process in plant growth and development The conserved Elongator histone H3 acetyl transferase complex and the HUB1 histone H2B monoubiquitination E3 ligase were identified by the leaf growth mutants they activate transcription by changing the chromatin state making the DNA template more accessible for the elongating RNA PII We study the upstream regulation target gene specificity downstream pathways and environmental interaction of these complexes Energy use in plants is an important component of yield stability in the field under varying environmental conditions Epilines were selected in canola and rice using energy use efficiency and seed yield in the field as parameters DNA hypo or hyper methylation was correlated with differential gene expression in order to study the epigenetic mechanisms underlying the phenotypes A maize transformation platform is at place for basic and translational research The transformation efficiency has been improved and the toolbox extended which allows a

    Original URL path: http://www.psb.vib-ugent.be/seminars/icalrepeat.detail/2015/04/30/151/-/genetic-and-epigenetic-components-in-plant-growth-and-crop-yield (2016-04-26)
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  • "How a monocot managed to live in the sea?"<br />"Mining the genome of the seagrass Zostera marina to figure it out"
    00am 12 30pm ABSTRACT Seagrasses are not really grasses but monocots within four families in the order Alismatales which are unique among angiosperms in having returned from land to the sea Forming large submerged meadows in coastal waters all over the world they are precious but endangered contributors to this environment To help maintaining these species and to figure out how this back to the sea event occurred the ca 500 Mb genome of Zostera marina has been sequenced and analyzed by a consortium led by Jeanine Olsen U Groningen in which our BEG annotation team has been a main player I will focus my presentation on what has been my own contribution to this task along two driving lines 1 Living under the sea Zostera probably can t use gasses the same way land plants do And indeed Zostera has lost stomata and all the genes encoding their differentiation as well as genes for the synthesis and sensing of ethylene and terpenoids 2 Flowering occurs in water too and pollination is hydrophilic Zostera has adapted to this fact by producing exine less and filiform pollen involving the loss of genes involved in the male development program Location Jozef Schell

    Original URL path: http://www.psb.vib-ugent.be/seminars/icalrepeat.detail/2015/05/07/153/-/how-a-monocot-managed-to-live-in-the-sea-br-mining-the-genome-of-the-seagrass-zostera-marina-to-figure-it-out (2016-04-26)
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  • "Ubiquitin and Ubiquitin-like proteins in plant stress signalling”
    Thursday 28 May 2015 11 00am 12 30pm ABSTRACT It is well established that the conjugation of Ubiquitin to target proteins is an important mechanism employed by plants and animals to perceive and respond to changing environments In recent years another class of ubiquitin related polypeptide tags called small ubiquitin like modifiers SUMO has emerged as a very influential regulator of stress signalling in plants and animals However very little information is available on SUMO proteins and their targets in plants We demonstrate that a proportion of DELLAs are conjugated to the Small Ubiquitin like Modifier SUMO protein the extent of conjugation increasing during stress We identify a SUMO interacting motif SIM in GID1 and demonstrate that SUMO conjugated DELLA binds to this motif in a GA independent manner The consequent sequestration of GID1 by SUMO conjugated DELLAs leads to an accumulation of non SUMOylated DELLAs and subsequent beneficial growth restraint during stress We conclude that plants have exploited SUMO to develop a GA independent mechanism to control growth during stress Location Jozef Schell seminar room Contact Dr Ari Sadanandom Director Durham Centre for Crop Improvement Technology School of Biological and Biomedical Sciences Durham University Durham United Kingdom Back PSB

    Original URL path: http://www.psb.vib-ugent.be/seminars/icalrepeat.detail/2015/05/28/156/-/ubiquitin-and-ubiquitin-like-proteins-in-plant-stress-signalling (2016-04-26)
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