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  • DNA Integrity Checkpoint Control
    of years Plants achieve this long lifespan because of their ability to constantly generate new cells at localized regions called meristems which hold the stem cell niches SCN Remarkably at the centre of the root SCN a group of mitotically inactive cells are found called the Quiescent Centre QC Contrary to their surrounding stem cells QC cells divide infrequently Because QC cells appear to be more DNA stress tolerant compared to their surrounding stem cells it has been speculated that QC cell division is used to repopulate damaged stem cell niches in such way contributing to the longevity of the stem cell niche Based on the recent identification of a rate limiting factor for QC cell division we apply single cell imaging profiling and sequencing techniques to analyse the DNA damage response in stem cells In particular our objectives are to establish a full understanding on why QC cells are more DNA stress resistant compared to non QC stem to identify the molecular components that drive QC cell division and to unravel the signalling components that activate QC cell division in response to stress Combined these results may form the basis for the development of novel innovative strategies to obtain stress resistant crops thus offering new perspectives towards plant breeding for growth improvement Wild type left versus checkpoint mutant right plants Publications Hu Z Cools T Kalhorzadeh P Heyman J De Veylder L 2015 Deficiency of the Arabidopsis helicase RTEL1 triggers a SOG1 dependent replication checkpoint in response to DNA cross links Plant Cell 27 149 161 Kalhorzadeh P Hu Z Cools T Amiard S Willing E M De Winne N Gevaert K De Jaeger G Schneeberger K White C I and De Veylder L 2014 Arabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability Plant Cell 26 3680 3692 Yi D Lessa Alvim Kamei C Cools T Vanderauwera S Takahashi N Okushima Y Eekhout T Yoshiyama K O Larkin J Van den Daele H Conklin P Britt A Umeda M and De Veylder L 2014 The Arabidopsis thaliana SIAMESE RELATED cyclin dependent kinase inhibitors SMR5 and SMR7 control the DNA damage checkpoint in response to reactive oxygen species Plant Cell 26 396 309 Heyman J Cools T Vandenbussche F Heyndrickx K S Van Leene J Vercauteren I Vanderauwera S Vandepoele K De Jaeger G Van Der Straeten D and De Veylder L 2013 ERF115 controls root quiescent center cell division and stem cell replenishment Science 342 860 863 Cools T Iantcheva A Weimer A K Boens S Takahashi N Maes S Van den Daele H Van Isterdael G Schnittger A De Veylder L 2011 The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress Plant Cell 23 1435 1448 Cools T Iantcheva A Weimer A K Boens S Takahashi N Maes S Van den Daele H Van Isterdael G Schnittger A and De Veylder L 2011 The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation

    Original URL path: http://www.psb.vib-ugent.be/cell-cycle/279-dna-integrity-checkpoint-control (2016-04-26)
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  • Diatom Cell Division
    light conditions in the oceans To this end we perform an in depth characterization of dsCYC2 CDK complex activation by combining genomics with biochemical assays Phaedactylum tricornutum Seminavis robusta The defining feature of diatom biology and key to understanding their evolutionary success is their haplo diploid life cycle controlled by a unique endogenous cell size reduction restitution strategy that is used as a clocking mechanism to switch resources to cell differentiation and sexual reproduction Yet very little is known about the regulatory mechanisms and signaling pathways underlying diatom cell division cell size control and sexual reproduction In collaboration with Prof Wim Vyverman and Prof Koen Sabbe UGent we aim to characterize mechanisms of diatom mating As a first objective we aim to unravel the mechanisms that determine the mating type of diatoms Secondly we aim to uncover the signaling cascades involved in the cell size dependent sexual differentiation and maturation and the sexual reproduction itself Our results will be useful for the cultivation and breeding of microalgae which are an enormous but largely untapped source of bio active compounds for the pharmaceutical food and cosmetics industries as well as metabolic pathways that may provision our future energy needs Publications Huysman M J J Tanaka A Bowler C Vyverman W De Veylder L 2015 Functional characterization of the diatom cyclin dependent kinase A2 as a mitotic regulator reveals plant like properties in a non green lineage BMC Plant Biol 14 15 86 Huysman M J J Vyverman W and De Veylder L 2014 Molecular regulation of the diatom cell cycle J Exp Bot 65 2573 2584 Huysman M J J Martens C Vyverman W and De Veylder L 2014 Protein degradation during the diatom cell cycle annotation and transcriptional analysis of SCF and APC C ubiquitin ligase genes in Phaeodactylum tricornutum

    Original URL path: http://www.psb.vib-ugent.be/cell-cycle/280-diatom-cell-division (2016-04-26)
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  • Mieke Van Lijsebettens
    B 9052 Gent BELGIUM Fax 32 0 9 33 13 809 Prev Projects Mieke Van Lijsebettens Group Members Group in Action Publications Mieke Van Lijsebettens Research Training Network CHIP ET Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens

    Original URL path: http://www.psb.vib-ugent.be/mieke-van-lijsebettens (2016-04-26)
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  • Group Members
    Group in Action Publications Mieke Van Lijsebettens Research Training Network CHIP ET Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab

    Original URL path: http://www.psb.vib-ugent.be/group-members-milij (2016-04-26)
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  • Group in Action
    Action Publications Mieke Van Lijsebettens Research Training Network CHIP ET Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab Wout Boerjan

    Original URL path: http://www.psb.vib-ugent.be/group-in-action-milij (2016-04-26)
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  • Introduction
    in growth and yield The chromatin state at RNA PII transcript elongation is an important regulatory process in plant growth and development The conserved Elongator histone H3 acetyl transferase complex and the HUB1 histone H2B monoubiquitination E3 ligase were identified by leaf growth mutants in a mutational analysis programme in Arabidopsis they activate transcription by changing the chromatin state making the DNA template more accessible for the elongating RNA PII We study the upstream regulation target gene specificity downstream pathways and environmental interaction of these complexes Energy use in plants is an important component of yield stability in the field under varying environmental conditions Epilines were selected in canola and rice using energy use efficiency and seed yield in the field as parameters Transcriptome methylome and histone modifications are analyzed in order to identify underlying putative epialleles and networks A maize transformation platform is in place for basic and translational research The transformation efficiency has been improved and the toolbox extended which allows a higher throughput of constructs and makes the system more versatile Next Projects Mieke Van Lijsebettens Group Members Group in Action Publications Mieke Van Lijsebettens Research Training Network CHIP ET Research Groups Alain Goossens Lab Ann Depicker

    Original URL path: http://www.psb.vib-ugent.be/chromatin-and-growth/282-introduction (2016-04-26)
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  • Chromatin in Growth
    Grasser K Transcript elongation factors shaping transcriptomes after transcript initiation Trends Plant Sci 19 717 726 2014 Van Lijsebettens M Dürr J Woloszynska M and Grasser K Elongator and SPT4 SPT5 complexes as proxy to study RNA Polymerase II transcript elongation control of plant development Proteomics 14 2109 2114 2014 Tsukaya H Byrne M E Horiguchi G Sugiyama M Van Lijsebettens M and Lenhard M How do housekeeping genes control organogenesis Unexpected new findings on the role of housekeeping genes in cell and organ differentiation J Plant Res 126 3 15 2013 Himanen K Woloszynska M Boccardi TM De Groeve S Nelissen H Bruno L Vuylsteke M Van Lijsebettens M Histone H2B monoubiquitination is required to reach maximal transcript levels of circadian clock genes in Arabidopsis Plant J 72 249 260 2012a Himanen K Boccardi T M De Rycke R Odeny O and Van Lijsebettens M Is HUB1 a hub for plant fitness Plant Sign Behav 7 12 1537 1540 2012b Kojima S Iwasaki M Takahashi H Imai T Matsumara Y Fleury D Van Lijsebettens M Machida Y And Machida C ASYMMETRIC LEAVES2 and Elongator a histone acetyltransferase complex mediate the establishment of polarity in leaves of Arabidopsis thaliana Plant Cell Physiol 52 1259 1273 2011 Bruno L Muto A Spadafora ND Iaria D Chiappetta A Van Lijsebettens M and Bitonti MB Multi probe in situ hybridization to whole mount Arabidopsis seedlings Int J Dev Biol 55 197 203 2011 Van Lijsebettens M and Grasser K The role of the transcript elongation factor FACT and HUB1 in leaf growth and the induction of flowering Plant Sign Behav 5 715 717 2010 Versées W De Groeve S and Van Lijsebettens M Elongator a conserved multitasking complex Mol Microbiol 76 1065 1069 2010 Nelissen H De Groeve S Fleury D Neyt P Bruno L Bitonti M B Vandenbussche F Van Der Straeten D Yamaguchi T Tsukaya H Witters E De Jaeger G Houben A and Van Lijsebettens M Plant Elongator regulates auxin related genes during RNA polymerase II transcription elongation Proc Natl Acad Sci USA 107 1678 1683 2010 Lolas IB Himanen K Grønlund JT Lyngaard C Houben A Melzer M Van Lijsebettens M and Grasser KD The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and functionally interacts with HUB1 2 Plant J 61 686 697 2010 Nelissen H Boccardi T M Himanen K Van Lijsebettens M Impact of histone modifications on transcriptional regulation and plant growth and development Crit Rev Plant Sci 26 243 263 2007 Falcone A Nelissen H Fleury D Van Lijsebettens M Bitonti B Cytological investigations of the Arabidopsis thaliana elo1 mutant give new insights into leaf lateral growth and elongator function Ann Bot 100 261 70 2007 Fleury D Himanen K Cnops G Nelissen H Boccardi T M Maere S Beemster G Anami S Neyt P Robles P Micol J L Inzé D Van Lijsebettens M The Arabidopsis homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root

    Original URL path: http://www.psb.vib-ugent.be/chromatin-and-growth/283-chromatin-in-growth (2016-04-26)
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  • Epilines
    epilines with distinct transcriptome and chromatin modification profiles supporting activation of stress response genes and networks and explaining the respiration phenotypes and the superior performance in the field Verkest et al 2015 We further focus on the epigenetic analyses of rice epilines selected for improved energy use efficiency and higher yield in the field Hence epilines with distinct characteristics can be selected from a pool of isogenic seeds implying that implementation of epigenetics in breeding is promising Selected references Verkest A Byzova M Martens C Willems P Verwulgen T Slabbinck B Rombaut D Van de Velde J Vandepoele K Standaert E Peeters M Van Lijsebettens M Van Breusegem F and De Block M Selection for Improved Energy Use Efficiency and Drought Tolerance in Canola Results in Distinct Transcriptome and Epigenome Changes Plant Physiol 168 1338 1350 2015 De Block M and Van Lijsebettens M Energy efficiency and energy homeostasis as genetic and epigenetic components of plant performance and crop productivity Curr Opin Plant Biol 14 275 282 2011 Prev Next Projects Mieke Van Lijsebettens Group Members Group in Action Publications Mieke Van Lijsebettens Research Training Network CHIP ET Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel

    Original URL path: http://www.psb.vib-ugent.be/chromatin-and-growth/284-epilines (2016-04-26)
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