archive-be.com » BE » V » VIB-UGENT.BE

Total: 357

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • Protein Complex Isolation
    followed by mass spectrometry AP MS on the other hand We have developed a unique AP MS platform for protein complex isolation from plant cells Complex purifications are performed through tandem affinity purification TAP from Arabidopsis cell suspension cultures or whole seedlings Co purified proteins are identified by mass spectrometry in close collaboration with the Proteomics Core Facility at VIB We have been able to isolate the T plate complex driving endocytosis in plants and the AN3 complex driving cell proliferation in leaf Besides our publications holds numerous examples of successful protein complex purifications for proteins present in diverse cellular pathways in different organelles or the cytosol both soluble or membrane associated More recently our attention has also moved towards the optimization of tools to isolate transient or very temporal protein protein interactions Prev Next Projects Geert De Jaeger Group Members Group in Action Publications Geert De Jaeger Supplementary data TAP fasta Datasets Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab

    Original URL path: http://www.psb.vib-ugent.be/functional-interactomics/254-protein-complex-isolation (2016-04-26)
    Open archived version from archive


  • Technology Transfer to Crop Species
    equally well in crop species such as corn or rice We have also started AP MS development in wheat The study of molecular interactions in cell cultures happens outside the context of development Moreover the small size of Arabidopsis thaliana hampers the access to sufficient biomass for flexible protein complex dissection in tissues of developing organs Crop species with their much bigger organs lend themselves much better for such an approach Based on a quantitative approach we were able to show the dynamics in protein complex composition of the AN3 complex in corn an important regulator of cell proliferation in a developing leaf Such data can deliver new approaches for the engineering of organ growth in crops Prev Next Projects Geert De Jaeger Group Members Group in Action Publications Geert De Jaeger Supplementary data TAP fasta Datasets Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab Wout Boerjan Lab About PSB

    Original URL path: http://www.psb.vib-ugent.be/functional-interactomics/257-tap-transfer-to-crop-species (2016-04-26)
    Open archived version from archive

  • Chromatin Complex Isolation
    epigenetic gene expression We are developing an in situ method to detect protein DNA interactions for a genomic region of interest under near to physiolocical conditions Many methods are available to identify genes targeted by a certain DNA binding protein including chromatin immunoprecipitation From a gene centered point of view however in which the aim is to identify proteins binding to a certain genomic region of interest mostly artificial approaches have been used so far including yeast one hybrid and electrophoretic mobility shift assay EMSA Experimental conditions in these artificial approaches are far from physiological causing false negative results Therefore we are developing an in situ method to detect protein DNA interactions for a genomic region of interest under near to physiolocical conditions Prev Projects Geert De Jaeger Group Members Group in Action Publications Geert De Jaeger Supplementary data TAP fasta Datasets Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab

    Original URL path: http://www.psb.vib-ugent.be/functional-interactomics/258-chromatin-complex-isolation (2016-04-26)
    Open archived version from archive

  • Ive De Smet
    B 9052 Gent BELGIUM Fax 32 0 9 33 13 809 Projects Ive De Smet Group Members Publications Ive De Smet Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere

    Original URL path: http://www.psb.vib-ugent.be/ive-de-smet (2016-04-26)
    Open archived version from archive

  • Group Members
    Ive De Smet Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab Wout Boerjan Lab About PSB PSB Missions The Department

    Original URL path: http://www.psb.vib-ugent.be/group-members-ivsme (2016-04-26)
    Open archived version from archive

  • Publications Ive De Smet
    Ive De Smet Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab Wout Boerjan Lab About PSB PSB Missions The Department

    Original URL path: http://www.psb.vib-ugent.be/publications-ive-de-smet (2016-04-26)
    Open archived version from archive

  • Introduction
    Annu Rev Cell Dev Biol 24 81 but there is an urgent need to gain insight in protein changes on different levels including protein protein interactions and post translational protein modifications With respect to the latter temporary and reversible phosphorylation of proteins is essential in regulating intracellular biological processes Phosphorylation affects protein folding conformation protein function and the regulation of enzymatic activities defines substrate specificity and influences protein localization complex formation and degradation The mechanistic importance of phosphorylation is obvious from its major influence on various cell functions such as signal transduction cell division cell differentiation and metabolic maintenance During the last ten years Arabidopsis thaliana has been proven to be an efficient model plant to study plant growth and development but time has come to investigate signaling cascades in crop species such as maize wheat and rice species that are currently also under investigation Next Projects Ive De Smet Group Members Publications Ive De Smet Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab

    Original URL path: http://www.psb.vib-ugent.be/functional-phosphoproteomics/263-introduction (2016-04-26)
    Open archived version from archive

  • Phosphatases and Kinases
    is controlled by a large number of protein kinases and phosphatase complexes plantsp genomics purdue edu family class html Dissmeyer and Schnittger 2011 Marshall et al 2012 However for the majority of cytoplasmic kinases membrane associated receptor kinases and phosphatases unraveling physiological and developmental roles and identifying substrates remain a challenge Recommended Reading Marshall A Aalen R B Audenaert D Beeckman T Broadley M R Butenko M A Caño Delgado A I de Vries S Dresselhaus T Felix G Graham N S Foulkes J Granier C Greb T Grossniklaus U Hammond J P Heidstra R Hodgman C Hothorn M Inzé D Østergaard L Russinova E Simon R Skirycz A Stahl Y Zipfel C De Smet I 2012 Tackling drought stress RECEPTOR LIKE KINASES present new approaches Plant Cell 24 2262 2278 Nakagami H Sugiyama N Ishihama Y Shirasu K 2012 Shotguns in the front line phosphoproteomics in plants Plant Cell Physiol 53 118 124 Bond A E Row P E Dudley E 2011 Post translation modification of proteins methodologies and applications in plant sciences Phytochemistry 72 975 996 Dissmeyer N Schnittger A 2011 The age of protein kinases Methods Mol Biol 779 7 52 Kline Jonakin K G Barrett Wilt G A Sussman M R 2011 Quantitative plant phosphoproteomics Curr Opin Plant Biol 14 507 511 de la Fuente van Bentem S Hirt H 2007 Using phosphoproteomics to reveal signalling dynamics in plants Trends Plant Sci 12 404 411 Prev Next Projects Ive De Smet Group Members Publications Ive De Smet Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere

    Original URL path: http://www.psb.vib-ugent.be/functional-phosphoproteomics/264-phosphatases-and-kinases (2016-04-26)
    Open archived version from archive