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  • Microscopy Infrastructure
    slit in front of the UV emission can be inserted in the microscope allowing fast photoconversion of photoswitchable fluorophores on a small spot or a line Available lenses include 10x 20x dry 40x and 63x water corrected Confocal LSM 5 Exciter Zeiss This upright confocal microscope has 405nm 458nm 488nm 561nm 633nm laser excitation lines Water dipping lenses allowing visualization without coverslips are available 20x 40x and 63x The microscope can be tilted horizontally to allow live visualization of specimens such as actively growing Arabidopsis roots within the gravity vector Available lenses include 10x 20x dry 25x 40x and 63x water corrected Confocal LSM510 Zeiss Pascal This inverted confocal microscope has 458nm 488nm 543nm 633nm laser excitation lines There are two filter based detectors and a DIC detector Available lenses include 10x 20x dry and 63x water corrected Spinning disc Ultraview Perkin Elmer This inverted Nikon Ti microscope based system has 405nm 440nm 488nm 515nm 561nm 640nm diode laser excitation lines controlled by the Volocity software Two Hamamatsu 512x512 EMCCD cameras and a 509 and 580 dichroic kit allow simultaneous imaging of GFP RFP or CFP YFP The microscope is equipped with a Perfect Focus System PFSIII reducing Z drift deal for long time imaging Available lenses include 10x 20x dry 60x water and 100x oil corrected When speed is of superior demand rather than resolution this system is the most convenient choice Ratiometric imaging Ca2 sensors phosphorylation sensors 4D stitching and photokinesis FRAP on fly are examples of the possibilities that this system offers Epifluorescence microscope Axiovert 135M Zeiss This is an inverted microscope with automated stage and shutters controlled by the Axiovision software ideal for generating multi area time lapses and high content screening purposes Filter sets are available for DAPI CFP GFP YFP RFP GFP RFP CFP YFP Several objective lenses up to 40x dry are available Available lenses include 10x 20x dry 40x dry and 40x oil corrected Epifluorescence microscope Axioimager M1 Zeiss This is an upright microscope with manual stage controlled by the Axiovision software and Zen blue Filter sets are available for DAPI GFP RFP CFP YFP CFP YFP FRET Several objective lenses up to 40x dry are available and also water dipping lenses 20x 40x 63x can fitted onto this microscope for screening purposes Available lenses include 10x 20x dry 40x dry and 40x oil corrected Epifluorescence microscope Axioskop Zeiss This is an upright microscope with manual stage Filter sets are available for DAPI CFP GFP YFP RFP GFP RFP CFP YFP Available lenses include 10x 20x dry and 40x oil corrected Macroscope MVX10 Olympus This microscope has been tilted horizontally to allow low magnification visualization of fluorescent markers during gravistimulation experiments It has automated shutters although it is still equipped with a manual stage This system allows to follow root growth during several up to 40 hours allowing to correlate fluorescent marker expression with root development and growth Filter sets are available for GFP YFP and RFP Available lenses include 1x and

    Original URL path: http://www.psb.vib-ugent.be/advanced-live-cell-imaging/230-microscopy-infrastructure (2016-04-26)
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  • Confocal Movies
    metaphase to cytokinesis movie courtesy of Daniël Van Damme Tobacco BY 2 cell expressing CycB1 1 GFP from prophase to late anaphase Note the fast degradation of this cyclin post metaphase movie courtesy of Joanna Boruc Merged confocal and DIC movie of a tobacco BY 2 cell expressing the end binding protein EB1c GFP from prophase to cytokinesis movie courtesy of Daniël Van Damme DIC and confocal movie of two tobacco BY 2 cells expressing KRP1 GFP from prophase to cytokinesis movie courtesy of Joanna Boruc Arabidopsis hypocotyl cell expressing GFP MBD showing microtubular dynamics movie courtesy of Daniël Van Damme Tobacco BY 2 cell stained with FM4 64 and silenced for NtTPLATE from prophase to cytokinesis Note the failure of the cell plate to anchor to the plasma membrane movie courtesy of Daniël Van Damme Arabidopsis epidermal root cells tml 1 tml 1 background expressing functional pTML TML GFP showing endocytic dynamicity each image taken every 500 msec Movie courtesy of Evelien Mylle Arabidopsis root cells expressing MAP65 1 TagRFP labeling microtubules in a growing root indicated with white arrow The movie shows a single plane in time made by stitching several frames per timepoint taken every 2 minutes using a spinning disc microscope Movie courtesy of Evelien Mylle Detail of the previous movie of arabidopsis root cells expressing MAP65 1 TagRFP processed with Volocity to correct for movement in order to follow two cells during cell division indicated with white arrows Movie courtesy of Evelien Mylle Physcomitrella patens cell expressing GFP tubulin and mRFP histon from prophase to cytokinesis Images are taken every 500 msec Scale 10 um Movie courtesy of Ken Kosetsu Prev Next Projects Daniel Van Damme Group Members Lab Members and Alumni Group in Action Web Links Publications Daniel Van Damme Research Groups Alain Goossens

    Original URL path: http://www.psb.vib-ugent.be/advanced-live-cell-imaging/231-confocal-movies (2016-04-26)
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  • Selected Group Publications
    U Swarup K De Smet I Van Damme D Lawrence T Péret B Moscardi E Barbeau D Godin C Salt D Guyomarc h S Stelzer E H K Maizel A Laplaze L and Bennett M J 2013 Lateral root morphogenesis is dependent on the mechanical properties of the overlaying tissues Proc Natl Acad Sci USA 110 5229 5234 Irani N G Di Rubbo S Mylle E Van den Begin J Schneider Pizo J Hniliková J Šíša M Buyst D Vilarrasa Blasi J Szatmári A M Van Damme D Mishev K Codreanu M C Kohout L Strnad M Caño Delgado A I Friml J Madder A and Russinova E 2012 Fluorescent castasterone reveals BRI1 signaling from the plasma membrane Nat Chem Biol 8 583 589 Van Damme D De Rybel B Gudesblat G Demidov D Grunewald W De Smet I Houben A Beeckman T and Russinova E 2011 Arabidopsis Aurora kinases function in formative cell division plane orientation Plant Cell 23 4013 4024 Drakakaki G Robert S Szatmari A M Brown M Q Nagawa S Van Damme D Leonard M Yang Z Girke T Schmid S L Russinova E Friml J Raikhel N V and Hicks G R 2011 Clusters of bioactive compounds target dynamic endomembrane networks in vivo Proc Natl Acad Sci USA 108 17850 17855 Eloy N B de Freitas Lima M Van Damme D Vanhaeren H Gonzalez N De Milde L Hemerly A S Beemster G T S Inzé D and Ferreira P C G 2011 The APC C subunit 10 plays an essential role in cell proliferation during leaf development Plant J 68 351 363 Van Damme D Gadeyne A Vanstraelen M Inzé D Van Montagu M C E De Jaeger G Russinova E and Geelen D 2011 Adaptin like protein TPLATE and clathrin recruitment during plant somatic cytokinesis occurs via two distinct pathways Proc Natl Acad Sci USA 108 615 620 Fache V Gaillard J Van Damme D Geelen D Neumann E Stoppin Mellet V and Vantard M 2010 Arabidopsis kinetochore fiber associated MAP65 4 cross links microtubules and promotes microtubule bundle elongation Plant Cell 22 3804 3815 Boruc J Mylle E Duda M De Clercq R Rombauts S Geelen D Hilson P Inzé D Van Damme D and Russinova E 2010 Systematic localization of the Arabidopsis core cell cycle proteins reveals novel cell division complexes Plant Physiol 152 553 565 Van Damme D 2009 Division plane determination during plant somatic cytokinesis Curr Opin Plant Biol 12 745 751 De Smet I Vassileva V De Rybel B Levesque M P Grunewald W Van Damme D Van Noorden G Naudts M Van Isterdael G De Clercq R Wang J Y Meuli N Vanneste S Friml J Hilson P Jürgens G Ingram G C Inzé D Benfey P N and Beeckman T 2008 Receptor like kinase ACR4 restricts formative cell divisions in the Arabidopsis root Science 322 594 597 Gaillard J Neumann E Van Damme D Stoppin Mellet V Ebel C Barbier E Geelen D and Vantard M

    Original URL path: http://www.psb.vib-ugent.be/advanced-live-cell-imaging/232-selected-group-publications (2016-04-26)
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  • Information for PhD Candidates
    Current vacancy Joint PhD position Functional Interactomics and Advanced Live Cell Imaging Prev Projects Daniel Van Damme Group Members Lab Members and Alumni Group in Action Web Links Publications Daniel Van Damme Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke

    Original URL path: http://www.psb.vib-ugent.be/advanced-live-cell-imaging/233-information-for-phd-candidates (2016-04-26)
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  • Collaborations
    Ghent Belgium Geert De Jaeger Functional Proteomics Plant Systems Biology Ghent Belgium Lieven De Veylder Cell Cycle Plant Systems Biology Ghent Belgium Tom Beeckman Root Development Plant Systems Biology Ghent Belgium Mark Stitt Metabolomics Max Planck Institute Golm Germany Gerrit Beemster Modeling University Antwerp Belgium Doris Wagner Chromatin Modeling University of Pennsylvania US Enrico Pè Maize Genetics Scuola Superiore Sant Anna Pisa Italy Isabel Roldán Ruiz Biomass ILVO Belgium Vasileios Fotopoulos Stress Tolerance Cyprus University of Technology Cyprus Paulo Ferreira and Adriana S Hemerly The Anaphase promoting complex Laboratório de Biologia Molecular de Plantas Instituto de Bioquímica Médica CCS Brasil Michael Muszynski Maize Leaf Development Dept of Genetics Development Cell Biology Iowa State University USA Prev Projects Yield Booster Website Collaborations Dirk Inzé Lab Members and Alumni Group Members Publications Dirk Inzé News Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab Wout Boerjan Lab About PSB PSB Missions The Department

    Original URL path: http://www.psb.vib-ugent.be/collaborations (2016-04-26)
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  • Dirk Inzé
    927 B 9052 Gent BELGIUM Fax 32 0 9 33 13 809 Prev Next Projects Yield Booster Website Collaborations Dirk Inzé Lab Members and Alumni Group Members Publications Dirk Inzé News Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van

    Original URL path: http://www.psb.vib-ugent.be/dirk-inze (2016-04-26)
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  • Lab Members and Alumni
    Yield headed by Prof Dirk Inzé He is involved in the design and establishment of several plant phenotyping platforms in the department giving support on the design and analysis of phenotyping experiments and supervising the development of the phenotyping data management software called PIPPA His current research project focuses on the identification and mode of action research of plant growth enhancing small molecules using an image based chemical genetics approach Predoctoral Researchers Jonas Blomme Predoctoral fellow Jonas Blomme obtained his Masters Degree in Biology at Ghent University in 2011 He joined the Systems Biology of Yield group during his master thesis which involved scoring the growth phenotype of mutants producing larger leaves on mild stress conditions He continued working in the lab of Dirk Inzé and started his PhD study with an IWT grant His research focus was initially screening potential new cell cycle regulators known to interact with core cell cycle regulators for leaf growth phenotypes His current work involves characterising proteins involved in mitochondrial gene expression in collaboration with the labs of Harvey Millar and Moussa Benhamed Kim Feys Predoctoral fellow Kim Feys received her Master degree in Biotechnology and Biochemistry in 2012 at Ghent University She did her master dissertation on the genetic variation in genes underlying cell wall digestibility in Lolium Perenne at ILVO Afterwards she joined the Systems Biology of Yield group focusing on the effects of heterosis on leaf growth in maize Ting Li Perdoctoral fellow Ting Li obtained her master degree of biology in Shanghai Jiaotong University in 2015 During her master study she focused on exploring the crosstalk between jasmonic acid and far red light on the regulation of anthocyanin accumulation In June 2015 she started her PhD in yield group at PSB Her project is mainly about the identification of new regulators of leaf growth under mild stress conditions in Arabidopsis Younjeong Nam Predoctoral fellow Younjeong Nam graduated in 2009 as Master in Molecular Cellular biology at Korea University in Korea with a thesis titled Characterization of tobacco IspH specifically interacting with cucumber mosaic virus encoded 2b protein She discovered that Japan had many more science related secrets waiting to be uncovered Therefore she moved to the RIKEN Plant Science Center Yokohama as a visiting researcher funded by the global internship program by the National Research Foundation of Korea where she studied on roles of cytokinin in low potassium signaling in Arabidopsis for 2 years Then Belgium beers got her interest so she quickly moved to Zwijnaarde and joined the lab of Dirk Inzé at the PSB to enthusiastically study the effect of transgenes in different Arabidopsis accessions Younjeong Nam graduated in 2009 as Master in Molecular Cellular biology at Korea University in Korea with a thesis titled Characterization of tobacco IspH specifically interacting with cucumber mosaic virus encoded 2b protein Then she moved to Japan at RIKEN Plant Science Center Yokohama as a visiting researcher funded by the global internship program by the National Research Foundation of Korea She was studying on roles of cytokinin in low potassium signaling in Arabidopsis for 2 years in Japan In 2011 she started her PhD at the lab of Dirk Inze at PSB to study about the effect of transgenes in different Arabidopsis accessions Judith Van Dingenen Predoctoral fellow I studied Biochemistry and Biotechnology at the University of Ghent and finished my Masters in 2011 with major Plant Biotechnology and minor Research During my thesis I investigated the role of strigolactones during nodulation and branching in different kinds of legumes Medicago red clover and pea For my Phd project I switched to Arabidopsis and my main focus is understanding how sugars as signalling molecules as well as the conserved glucose sensor HEXOKINASE1 HXK1 regulate early leaf development Additionally I work together with Chrystalla Antoniou visiting scientist of Cyprus University on strobilurin compounds which are agrochemical fungicides that also demonstrate plant growth promoting features Lennart Verbraeken Predoctoral fellow Lennart Verbraeken obtained his master s degree in Bioscience engineering in 2014 at Ghent University For his master s thesis he researched the water and carbon relations in a filling wheat ear using portable NMR technology in cooperation with the Forschungszentrum Jülich in Germany and under supervision of prof dr ir Kathy Steppe dr Carel Windt and dr ir Veerle De Schepper He joined PSB in 2015 to start his PhD research on the growth and physiology of maize growing under drought conditions using the Phenovision phenotyping platform Jasmien Vercruysse Predoctoral fellow Jasmien graduated in 2014 as a Master of Science in bioinformatics after completing her master thesis which focussed on the rewiring of the plant interactome in response to environmental stress She quickly discovered that the wonderful world of computational science could be complemented by experimental lab work That is why she joined the Systems Biology of Yield group where she is currently doing a phd in which both computational and experimental approaches harmoniously blend Her work includes designing and optimizing synthetic promoters that create specific expression profiles in both Arabidopsis and maize Xiaohuan Sun Predoctoral fellow Xiaohuan Sun graduated as Master in Plant Genetic Breeding from Sichuan Agricultural University in 2011 and subsequently joined the group of Dirk Inzé for a PhD Her research focuses on the molecular mechanisms that define the transition zone between cell division and cell expansion in standard and mild water deficit conditions Alexandra Baekelandt Predoctoral fellow Alexandra Baekelandt graduated in 2013 as Master of Science in Biochemistry and Biotechnology at Ghent University major Plant Biotechnology She first joined the Systems Biology of Yield group for her master dissertation entitled Meristemoid cell division and leaf growth under standard conditions based on understanding how the division of epidermal stem cell like cells called meristemoids influences leaf growth and which molecular players are involved in this process After that she got an IWT grant and started in January 2014 a PhD where she mainly continues the research done during his master dissertation Michiel Bontinck Predoctoral fellow Michiel graduated as a Master of

    Original URL path: http://www.psb.vib-ugent.be/lab-members-and-alumni-diinz (2016-04-26)
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  • Group Members
    Inzé Lab Members and Alumni Group Members Publications Dirk Inzé News Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab Wout

    Original URL path: http://www.psb.vib-ugent.be/group-members-diinz (2016-04-26)
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