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  • Group Members
    Members Lab Members and Alumni Group in Action Web Links Publications Daniel Van Damme Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de

    Original URL path: http://www.psb.vib-ugent.be/group-members-dadam (2016-04-26)
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  • Lab Members and Alumni
    the Arabidopsis core cell cycle proteins through investigation of their subcellular localization and the binary interactions that take place among them Subsequently Joanna move to Iris Meier s lab at the Ohio State University USA where she was investigating the requirement of plant RanGAP1 subcellular targeting and GAP activity for its cellular and developmental functions 2010 2012 After having received The Back to Belgium Grants of the federal Science Policy BELSPO she has been elucidating the involvement of Arabidopsis alpha aurora kinases in lateral root formation under the supervision of Daniël Van Damme Ken Kosetsu Postdoctoral fellow Ken Kosetsu obtained his PhD at Nagoya university Japan in 2011 The work was performed under the supervision of Dr Yasunori Machida During his PhD he focused on mechanisms of the expansion of the phragmoplast MTs during cytokinesis in A thaliana Subsequently he moved to the Gohta Goshima lab at the Nagoya University where he was investigating the role of MAP65 in mitosis in P patens 2011 2015 In 2015 he joined the Advanced Live Cell Imaging Group as a postdoctoral fellow where he is now working on asymmetric cell division determination using A thaliana and P patens as model systems Predoctoral Researchers Klaas Yperman Predoctoral fellow Klaas obtained a Master in Biochemistry Biotechnology at Ghent University in 2015 He did his master II thesis in the lab of Dr Jerry Ståhlberg and Dr Mats Sandgren at SLU in Sweden where he focused on the binding of cellulose degrading enzymes LPMOs onto different substrates In October 2015 he started as a PhD student in the ALICE group working on the structural elucidation of the TPLATE complex under the guidance of Dr Daniël Van Damme Jie Wang Predoctoral fellow Jie obtained his Master degree in Pesticides at South China Agricultural university China 2015 under the supervision of Professor Hanhong Xu His master project mainly focuses on investigating the phloem mobility of glucose pesticides and visualizing their translocation in Castor bean In October 2015 he joined the Advanced Live Cell Imaging Group as a PhD student working on the dynamic recruitment at the plasma membrane and the functional characterization of the different TPLATE complex subunits under the supervision of Dr Daniël Van Damme Technical Staff Evelien Mylle Lab manager Evelien Mylle graduated in 2001 as Industrial engineer specialization biochemistry at the Provinciale Industriele Hogeschool PIH in Kortrijk Belgium After working 6 months at the Laboratory of Molecular Biology DMBR at Gent University she moved to Brussels to work at the Scientific Institute of Public Health IPH section Biotechnology and Biosafety SBB to trace genetic modified organisms in food Since August 2004 she joined the former Cell Biology Group Prof Danny Geelen at PSB From 2006 this group changed to the Brassinosteroids group under the supervision of Dr Eugenia Russinova where she contributed to study the Brassinosteroid pathway mainly by using fluorescence microscopy techniques In order to become more specialized in the microscopy field she moved from January 2015 to the Advanced Live Cell Imaging group

    Original URL path: http://www.psb.vib-ugent.be/lab-members-and-alumni-dadam (2016-04-26)
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  • Group in Action
    Alumni Group in Action Web Links Publications Daniel Van Damme Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab Wout Boerjan

    Original URL path: http://www.psb.vib-ugent.be/group-in-action-dadam (2016-04-26)
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  • Web Links
    of GFP tagged proteins Plant J 40 386 398 The Illuminated Plant Cell Next Projects Daniel Van Damme Group Members Lab Members and Alumni Group in Action Web Links Publications Daniel Van Damme Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab

    Original URL path: http://www.psb.vib-ugent.be/web-links (2016-04-26)
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  • Publications Daniel Van Damme
    Members Lab Members and Alumni Group in Action Web Links Publications Daniel Van Damme Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de

    Original URL path: http://www.psb.vib-ugent.be/publications-daniel-van-damme (2016-04-26)
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  • Introduction
    elucidation and profound understanding of the mechanisms at play which orient and subsequently maintain the division plane To do so we are combining state of the art cell biological genetic and proteomic approaches using Arabidopsis thaliana tobacco BY 2 and Physcomitrella patens as model organisms Merged DIC and confocal time lapse sequence of a tobacco BY 2 cell expressing the microtubule binding domain of MAP4 fused to RFP The cell divides and shows the PPB degradation followed by spindle and phragmoplast formation The phragmoplast builds the cell plate expands outward and guides the cell plate via an unknown mechanism to the former position of the PPB Where mainly simple geometric rules apply to determine the position of the future division plane in proliferative divisions Besson and Dumais 2011 formative divisions require additional yet largely unknown internal or external cues to determine their orientation De Smet and Beeckman 2011 To elucidate the mechanism of division plane orientation in both proliferative and formative divisions we are working on several key players involved in various steps of this process We are working on PPB formation through the PP2A phosphatase holo enzyme complex which includes TON1 and FASS and is targeted to the microtubular cytoskeleton via the TRM proteins Spinner et al 2013 we are addressing the identity maintenance of the CDZ via elucidating the dynamic behavior and interplay between positive CDZ markers we are looking into the function of the aurora kinase family in division plane orientation as the alpha group of aurora kinases plays a major role in orienting formative divisions from embryogenesis onward Van Damme et al 2011b and we are looking into the role of membrane trafficking events at the PPB taking advantage of the identification of the TPLATE complex as a predominant factor for clathrin mediated endocytosis at the plasma membrane Gadeyne et al 2014 References Besson S and Dumais J 2011 Universal rule for the symmetric division of plant cells Proc Natl Acad Sci USA 108 6294 6299 De Smet I and Beeckman T 2011 Asymmetric cell division in land plants and algae the driving force for differentiation Nat Rev Mol Cell Biol 12 177 188 Gadeyne A Sánchez Rodríguez C Vanneste S Di Rubbo S Zauber H Vanneste K Van Leene J De Winne N Eeckhout D Persiau G Van De Slijke E Cannoot B Vercruysse L Mayers JR Adamowski M Kania U Ehrlich M Schweighofer A Ketelaar T Maere S Bednarek SY Friml J Gevaert K Witters E Russinova E Persson S De Jaeger G Van Damme D 2014 The TPLATE adaptor complex drives clathrin mediated endocytosis in plants Cell 156 691 704 Karahara I Suda J Tahara H Yokota E Shimmen T Misaki K Yonemura S Staehelin L A and Mineyuki Y 2009 The preprophase band is a localized center of clathrin mediated endocytosis in late prophase cells of the onion cotyledon epidermis Plant J 57 819 831 Rasmussen C G Humphries J A and Smith L G 2011 Determination of symmetric and asymmetric division

    Original URL path: http://www.psb.vib-ugent.be/advanced-live-cell-imaging/227-introduction (2016-04-26)
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  • Aurora Kinases and Division Plane Determination
    for formative divisions provides an ideal genome wide screening system to identify novel players involved in division plane determination Identified candidate proteins will subsequently be characterized genetically and biochemically Currently we are addressing the role of aurora dependent phosphorylation of the microtubule bundling protein MAP65 1 as well as other candidate substrate proteins both in Arabidopsis and Physcomitrella patens We are performing an EMS based screen in Arabidopsis to find revertants of the aurora double knockdown mutant phenotype and are using and developing proteomics tools phosphoproteomics TAP tag and bioID assays to identify novel interactors as well as substrates of this family of kinases Time lapse movie of an AURORA1 GFP expressing tobacco BY 2 cell going through mitosis AURORA1 labels the prophase spindle kinetochore spindle microtubules and the cell plate Time lapse movie of initial lateral root primordium pericycle cell divisions in an Arabidopsis double mutant for aurora 1 and aurora 2 aur1 2 aur2 2 expressing PIN1 GFP as a membrane marker Whereas these initial divisions switch between anticlinal and periclinal orientations in a wild type lateral root primordium the divisions in the aurora double mutant are oblique and lead to an unstructured primordium lacking defined cell layers Initial lateral root primordium divisions marked by PIN1 GFP in a wild type root left and in an aurora double mutant root right Whereas the initial wild type lateral root divisions are asymmetric and anticlinal the divisions in the aurora double mutant root are oblique to periclinal References Van Damme D De Rybel B Gudesblat G Demidov D Grunewald W De Smet I Houben A Beeckman T and Russinova E 2011 Arabidopsis alpha Aurora kinases function in formative cell division plane orientation Plant Cell 23 4013 4024 Prev Next Projects Daniel Van Damme Group Members Lab Members and Alumni Group

    Original URL path: http://www.psb.vib-ugent.be/advanced-live-cell-imaging/228-aurora-kinases-and-division-plane-determination (2016-04-26)
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  • The TPLATE Complex and Endocytosis
    specific TPLATE complex the AP 2 complex and its their cargo will allow modulation of the internalization of certain cargo proteins including those involved in division plane determination and identity maintenance Identification of TPLATE complex and AP 2 complex cargo proteins will be performed using various approaches including Two hybrid co IP and Tandem affinity purification targeted biotinylation bioID Roux et al 2012 where a promiscuous biotin ligase will cause biotinylation of interactors of both adaptor complexes Biotinylated proteins will subsequently be purified and identified using mass spectrometry Next to this approach we are evaluating the function of several domains present in TPLATE complex subunits by performing mutant complementation experiments and we are dissecting the cause of the male sterility of TPLATE complex subunit mutants using 3D scanning electron microscopy Time lapse movie of two tobacco BY 2 cells expressing TPLATE GFP and RFP TUA2 from metaphase to the end of cytokinesis TPLATE GFP accumulates strongly to the expanding cell plate and labels the CDZ during the insertion of the cell plate References Gadeyne A Sánchez Rodríguez C Vanneste S Di Rubbo S Zauber H Vanneste K Van Leene J De Winne N Eeckhout D Persiau G Van De Slijke E Cannoot B Vercruysse L Mayers JR Adamowski M Kania U Ehrlich M Schweighofer A Ketelaar T Maere S Bednarek SY Friml J Gevaert K Witters E Russinova E Persson S De Jaeger G Van Damme D 2014 The TPLATE adaptor complex drives clathrin mediated endocytosis in plants Cell 156 691 704 Hirst J Schlacht A Norcott JP Traynor D Bloomfield G Antrobus R Kay RR Dacks JB Robinson MS 2014 Characterization of TSET an ancient and widespread membrane trafficking complex Elife 3 e02866 Roux KJ Kim DI Raida M Burke B 2012 A promiscuous biotin ligase fusion protein identifies

    Original URL path: http://www.psb.vib-ugent.be/advanced-live-cell-imaging/229-the-tplate-complex-and-endocytosis (2016-04-26)
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