archive-be.com » BE » V » VIB-UGENT.BE

Total: 357

Choose link from "Titles, links and description words view":

Or switch to "Titles and links view".
  • Combinatorial Biochemistry & Synthetic Biology
    compounds The ultimate aim is to create a rainforest like metabolic diversity within a few performing organisms in the laboratory which will allow launching a new era of bioprospecting of the metabolic richness of the plant kingdom Triterpene saponin producing plant species Synthesis of novel molecules will be achieved through combinatorial biosynthesis which involves interchanging specializedsecondary metabolism genes between organisms to create unnatural hitherto unexisting gene combinations or hybrid genes Novel metabolites can be made due to the effect of new enzyme metabolic pathway combinations or due to the formation of proteins with new enzymatic properties Thus far an in vivo combinatorial biosynthesis approach has not been extended to higher plant systems or for plant derived molecules Our technology platform offers the opportunity to browse into the entire metabolic repertoire of a plant and to assess the principle of combinatorial biosynthesis both in whole plants and in plant cells The target plants that are employed are different medicinal plants all producing triterpene sapo ge nins amongst others the model plant Medicago truncatula and the medicinal plants Glycyrrhiza glabra Panax ginseng Bupleurum falcatum Maesa lanceolata Chenopodium quinoa Catharantus roseus Artemisia annua Platycodon grandiflorus Centella asiatica Tripterygium wilfordii and Aquilegia coerulea In parallel the enzyme encoding gene collection that is established is being employed to set up a synthetic biology platform for the synthesis of bioactive triterpenes and building blocks thereof in yeast cells Strategies to generate novel triterpene saponins Selected Publications Moses T Pollier J Almagro L Buyst D Van Montagu M Pedreño M A Martins J C Thevelein J M and Goossens A 2014 Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C 16 hydroxylase from Bupleurum falcatum Proc Natl Acad Sci USA 111 1634 1639 Moses T Pollier J Thevelein J M and Goossens A 2013

    Original URL path: http://www.psb.vib-ugent.be/specialized-metabolism/214-combinatorial-biochemistry-synthetic-biology (2016-04-26)
    Open archived version from archive


  • Plants and Human Health: Tomato as a Model
    abundant health depends on a balanced diet The latter depends amongst others on grains vegetables and fruits that are produced safely but also on sufficient intake of vitamins and minerals and access to health promoting molecules Therefore research on plant metabolic pathways is needed to produce food sources or plant derived products for the benefit of public health Within the frame of this theme we are launching a research program in the crop plant tomato to understand the processes that drive accumulation of pigments flavours vitamins nutraceuticals and bioactive terpenoids and to study the interface between primary and specialized metabolism during growth development and stress situations This research will also involve translation to the relevance for public health and Flemish agriculture and industry through collaboration with ILVO Prev Projects Alain Goossens Group Members Lab Members and Alumni Group in Action Publications Alain Goossens Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer

    Original URL path: http://www.psb.vib-ugent.be/specialized-metabolism/410-plants-and-human-health-tomato-as-a-model (2016-04-26)
    Open archived version from archive

  • Group Members
    and Alumni Publications Ann Depicker Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab Wout Boerjan Lab About PSB PSB Missions

    Original URL path: http://www.psb.vib-ugent.be/group-members-anpic (2016-04-26)
    Open archived version from archive

  • Lab Members and Alumni
    focus on the molecular regulation and the physiological role of endoreduplication in plants In 2013 she joined the Plant made Antibodies and Immunogens group of Prof A Depickeras an assistant Professor Predoctoral Researchers Jorge Palaci Bataller Predoctoral fellow Jorge Palaci Bataller graduated in 2013 as a Master in Biotechnology at the Polytechnic University of Valencia UPV in Spain During his MSc project he specialized on the GoldenBraid DNA cloning system for the evaluation of plant promoters and terminators in the research group of Prof D Orzaez IBMCP Valencia Spain Also in 2013 he joined the Plant made Antibodies and Immunogens group of Prof A Depicker as PhD student to work on the in seed production of antibodies and vaccines and the development of a strategy for the prevention of the piglet post weaning diarrhea disease Shruti Bakshi Predoctoral fellow Shruti Bakshi obtained her Bachelor in Biotechnology in Devi Ahilya University India and her Master degree in Biotechnology in Pune University India She did her Master s project Identification of biomarkers for breast cancer at the National Centre for Cell Science Pune After her Master she worked as a research fellow at Delhi University India and focused on developing diagnostic tests for infectious diseases She joined Prof Ann Depicker s group as a pre doctoral fellow in June 2015 Her main interest is to investigate the role of monomeric and secretory IgA for protection against human respiratory syncytial virus at the pulmonary mucosal surface in comparison with the existing IgG treatments in collaboration with Prof Xavier Saelens and Dr Bert Schepens of the UGent department Biomedical Molecular Biology and VIB department Biomedical Biotechnology Shruti Bakshi is also involved in another project focused on targeting vaccine molecules to elicit potent intestinal immunity protection against enteropathogens in the piglet model in collaboration with Prof E Cox of the UGent laboratory of Immunology Ashuwini THARAD Predoctoral fellow Ashuwini Tharad obtained her Bachelor s degree in Industrial Microbiology from the Abasaheb Garware College Pune India and Master s degree in Virology from the National Institute of Virology Pune India She did her Master s project in Analysis of the Antibody Profile of Dengue Virus Infected Individuals using Recombinant Core protein in the Dengue research group of Dr Cecilia Dayaraj NIV Pune She has worked as a Junior Research Assistant at Actis Biologics Pvt Ltd Mumbai She joined Prof Ann Depicker s group as a predoctoral fellow in August 2015 Her main interest is to develop a plant seed expression system for the production of candidate vaccine molecules and to evaluate their efficacy to elicit potent intestinal mucosal immunity for protection against porcine enteropathogens Technical Staff Jonah Nolf Lab manager Jonah Nolf obtained the degree of professional Bachelor in Biomedical Laboratory Techniqueswith specialization in Pharmacy and Biotechnologyat KAHO Sint Lieven Ghent Belgium in 2006 Following his studies he engaged himself to a position in the Plant made Antibodies and Immunogens group of Prof A Depicker and has been working there ever since Over the years he

    Original URL path: http://www.psb.vib-ugent.be/lab-members-and-alumni-details-anpic (2016-04-26)
    Open archived version from archive

  • Publications Ann Depicker
    and Alumni Publications Ann Depicker Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank Van Breusegem Lab Geert De Jaeger Lab Ive De Smet Lab Jenny Russinova Lab Lieven De Veylder Lab Mieke Van Lijsebettens Lab Moritz Nowack Lab Sofie Goormachtig Lab Steven Maere Lab Tom Beeckman Lab Yves Van de Peer Lab Wout Boerjan Lab About PSB PSB Missions

    Original URL path: http://www.psb.vib-ugent.be/publications-ann-depicker (2016-04-26)
    Open archived version from archive

  • Introduction
    been successfully used to produce diagnostic and therapeutic antibodies and vaccines To obtain full advantage of the seed production system it is essential to constantly evaluate its limitations and develop novel technologies to overcome the remaining obstacles Hence we also focus on optimizing this technology for example the best strategy to produce the complex secretory IgA is currently being worked out and also a generic protocol for purification of IgG and IgA antibodies For screening of new immunoglobulin variants and fusions we are using the Nicotiana benthamiana transient expression system because it is very flexible and up to milligram amounts can be obtained within one week To obtain recombinant protein for proof of concept studies we are employing Arabidopsis thaliana seeds as experimental recombinant protein production system because of its ease low workload and speed to go from DNA construct to 100 mg of recombinant protein in about one year For cost effective production of bulk amounts of complex recombinant proteins as is for instance needed for passive immunization we are exploring the use of larger protein rich seed crops such as soybean and pea The capacity to conveniently produce milligram to gram amounts of novel therapeutic proteins provides a unique platform for addressing question of animal and public health importance right from fundamental to applied level We focus on therapeutic antibodies specific vaccines and biotechnological tools Towards a valorization objective we are exploring the passive immunization approach in weaned piglets against the enterotoxigenic Escherichia coli together with the Cox lab Laboratory of Immunology Ghent University together with Sam Millet ILVO we are analyzing how colostrum and milk protects the suckling piglets and how mucosal immunity develops in weaned piglets At fundamental level together with the Saelens lab and Schepens Unit of Molecular Virology VIB Ghent University we are evaluating

    Original URL path: http://www.psb.vib-ugent.be/plant-made-antibodies/218-introduction (2016-04-26)
    Open archived version from archive

  • Plant-Made Antibodies for Prophylaxis
    Virdi et al 2013 In this work anti ETEC F4 antibodies were engineered from phage display selected variable domains from Henri de Greve s lab in the department of structural Biology in Brussels in a simplified IgA sIgA format These antibodies were then produced in seeds of Arabidopsis thaliana and scaled up to test their potential in passive immunization assays in vivo For this crushed seeds were incorporated in the feed of weaned piglets and showed to be protective against ETEC F4 challenge Following the same rationale antibodies against ETEC F18 are currently being developed In addition we plan to evaluate the stability of the antibodies during feed processing mimicking the conditions to which seeds are subjected in the common feed processing chain Also the functionality of the orally fed antibodies in fecal matter will be studied Evaluation of the presence and functionality of the plant made antibodies in the fecal samples will allow us to determine the stability during gut transit Elite antibodies against both F4 and F18 ETEC strains are currently being transformed and or scaled up in soya for further valorization see section 1 Development of maternal and piglet anti ETEC immunity In most commercial farms sows are vaccinated against enterotoxigenic Escherichia coli ETEC including F4 ETEC This immunization is essential to stimulate the production of specific antibodies and their transfer to the piglets via colostrum and milk to prevent diarrhea during the neonate period and shortly after weaning By determining and quantifying the different isotypes of ETEC specific antibodies in sow s blood serum and colostrum milk and the blood serum and stool of their suckling piglets before and after weaning we aim to obtain a better basic understanding of the transfer and role of the maternal protective antibodies Moreover it will help us evaluate the antibody dosage requirement for the recombinant antibody feed formulation Passive immunization against the human respiratory syncytial virus Delivery of antibodies at the mucosal surfaces as passive immunotherapeutic agents is a promising strategy to prevent and treat infectious diseases Human Respiratory Syncytial Virus HRSV is one of the major causes of lower respiratory tract infections in infants HRSV can cause severe disease and even death in frail individuals such as very young children and the elderly Prophylactic treatment with an HRSV targeting monoclonal antibody is clinically approved However the high cost that is associated with the current mammalian cell based manufacturing systems hampers the widespread implementation of this therapy Alternative expression platforms such as transient expression in plants not only provide higher scalability and reduced costs but also permit a rapid evaluation of different antibody versions In this work we will evaluate the efficacy of twelve different anti HRSV antibody variants produced in Nicotiana benthamiana leaves Antibodies against HRSV have been built by the combination of three different Nanobodies VHH Schepens et al 2011 genetically fused to the Fc fragment of different murine and human monomeric IgA and IgG antibodies Furthermore IgA based antibodies will also be expressed and tested in

    Original URL path: http://www.psb.vib-ugent.be/plant-made-antibodies/221-in-seed-antibody-production (2016-04-26)
    Open archived version from archive

  • Development of Mucosal Vaccines
    is a selectively permeable membrane were transcytosed antigens can either induce an immune reaction or a tolerogenic reaction Among parameters specific targeting to immune cells is important for mucosal immune response In a project together with Dr Cox Immunology Lab UGent and Dr Sanders Lab of Genetherapy UGent different recombinant mucosal vaccines will be produced in plants The Cox lab recently identified a receptor for protein transcytosis on the enterocytes of the small intestine Antibodies against these receptors were transcytosed across the small intestine gut lining and resulted in elicitation of specific immune response We will produce fusions between a monoclonal antibody against this receptor and different antigens in plant expression platforms starting with the green fluorescent reporter as model antigen Fusion vaccine with antibodies targeting an enterocyte receptor will allow evaluating transcytosis across the enterocytes and interaction with immune cells in vitro In a second phase delivery of the recombinant vaccines within the feed will allow to study the in vivo systemic and mucosal immune responses Prev Next Projects Ann Depicker Group Members Lab Members and Alumni Publications Ann Depicker Research Groups Alain Goossens Lab Ann Depicker Lab Bruno Cammue Lab Daniel Van Damme Lab Dirk Inzé Lab Frank

    Original URL path: http://www.psb.vib-ugent.be/plant-made-antibodies/418-development-of-mucosal-vaccines (2016-04-26)
    Open archived version from archive